Experiment number 1:
To use Thiobacilli cultured from the soil samples from walk 1 on Marsden moor to generate enough acid to etch into copper sheet.
The soil samples have been mixed with ground sulphur as a food source for the bacteria and are currently on the shaker in the lab to see what happens. Initial Ph testing of the samples has shown them to be between 4.2 and 5.5, a lot lower than was expected and low enough to cause possible difficulties in seeing the effects of the Thiobacillus. Anyhow, there is still a chance that they’ll pull the Ph further down to around 2 or 3 and we’ll be able to confirm their presence and do something with them. Otherwise I’ll just go and get some new samples from more reliable Thiobacilli rich areas.
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Ph testing

flasks on the shaker

Experiment number 2:
To use calcium precipitating bacilli species to lay down tracks of hardened calcite.
We aim to isolate and culture these bacilli (rod shaped bacteria) from the soil samples from walk 1 and immobilise them in alginate, a reaction between Sodium alginate and Calcium chloride. The cultured bacteria will be mixed into the sodium alginate and mixed with Calcium chloride which rapidly produces the thick jelly like substance alginate. Using a fine syringe we can lay this into tracks which will eventually be turned into stone like calcite but there is also the possibility of creating cast 3D objects. Alongside creating some nice stone visualisations of the landscape I quite like the idea of models of Pioneer, Voyager, Sojourner and the Arecibo dish made by moorland microbes.
Experiment number 3:
To encode the data and drawings produced by the bacteria in quaternery (4 base) and synthesise it into DNA.
The information and ‘cultural’ activities generated by the bacteria can be synthesised into DNA and placed into an E Coli carrier. Using basic bacterial transformation techniques the new DNA can be spliced into the DNA of the bacteria and cultured on form there. I’d love to build a mobile robot that presents the data and images on its screen while carrying a sort of time capsule containing all the data encoded into the genetic make up of the E Coli.
The problem with this experiment is that its pretty expensive, time consuming and notoriously hit and miss outside of expert labs. We’d be looking at spending around £500 for enough kit to have 40 goes at making it work without the guarantee that it would. Might have to leave this one on the back burner for a while. At the very least, though, I should be able to encode the data into quaternery and work out the DNA base pairs from there – and possibly even find out if there are any functioning genes hidden away within them.